Antithrombin Effect of Jidabokuippo and Identification of Active Compounds

Static blood (Oketsu) is a disorder of Kampo medicine. Many natural medicines have been used to cure static blood, and these drugs are called stasis-resolving formula. Some were reported inhibit coagulation. Jidabokuippo (JDI), one the formulas, has treat bruises sprains. In this study, we evaluated antithrombin activity JDI its active compounds using thrombin an artificial substrate. Bioassay-guided isolation was performed for JDI, identified spectral data (NMR spectroscopy mass spectrometry) by comparison with data. depressed in dose-dependent manner, ranging from 0.125 1 mg/mL. Chrysophanol-1- O-β-D-glucoside (1) chrysophanol-8- (2) obtained showed inhibitory activity. This study provided first report effect it two (1 2). These findings might contribute understanding mechanism